| 质粒名称: | pCMV2-SEP-GluA1 (M1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 64942 |
| 存储实验室: | Jeremy Henley |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV2 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GluA1 |
| 物种来源: | R. norvegicus (rat) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | SEP (superecliptic pHluorin) （插入物上的N端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | hGH-pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV2-SEP-GluA1 (M1) was a gift from Jeremy Henley (Addgene plasmid # 64942 ; http://n2t.net/addgene:64942 ; RRID:Addgene_64942) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pCMV2-SEP-GluA1 (M1) 8314 bp ds-DNA circular SYN 25-JUN-2020
DEFINITION mammalian expression of rat GluA1.
ACCESSION .
VERSION .
KEYWORDS pCMV2-SEP-GluA1 (M1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8314)
TITLE Jeremy Henley Plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8314)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 25, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8314
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 11..33
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 25..42
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 26..42
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
enhancer 203..582
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 583..786
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 737..757
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 969..1679
/codon_start=1
/product="pH-sensitive mutant of green fluorescent protein
(Ng et al., 2002)"
/label=superecliptic pHluorin
/note="excited strongly by blue light at neutral pH but
minimally fluorescent at acidic pH"
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
primer_bind complement(996..1024)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 1619..1639
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
polyA_signal 4367..4989
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(4566..4585)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
primer_bind complement(5013..5033)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5018..5347
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5198..5333
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5260..5279
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 5386..5482
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
primer_bind complement(5558..5577)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5559..5577)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5591..5607)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5591..5607)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5604..5626)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5615..5631
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5639..5669)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5684..5705
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5822..5839)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5993..6581)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6073..6092)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6752..7612)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7375..7394
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7613..7717)
/gene="bla"
/label=AmpR promoter
rep_origin 7744..8199
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7831..7850)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8041..8062
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct gatctataca
61 ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt
121 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
181 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
241 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
301 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
361 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
421 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
481 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
541 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
601 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
661 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
721 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
781 agagctcgtt tagtgaaccg tcagaattca gatctggtac cacgcgtatc gataagcttg
841 atatcgcttc ctggtgaagt gtgtttcttg aaatcatcac caccatggac agcaaaggtt
901 cgtcgcagaa agggtcccgc ctgctcctgc tgctggtggt gtcaaatcta ctcttgtgct
961 ctgcagctag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
1021 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
1081 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
1141 cacttgtcac tactctgacc tatggtgttc aatgcttttc aagataccca gatcatatga
1201 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
1261 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
1321 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
1381 acaaattgga atacaactat aacgatcacc aggtgtacat catggcagac aaacaaaaga
1441 atggaatcaa agctaacttc aaaattagac acaacattga agatggaggc gttcaactag
1501 cagaccatta tcaacaaaat actccaattg gcgatgggcc cgtcctttta ccagacaacc
1561 attacctgtt tacaacttct actctttcga aagatcccaa cgaaaagaga gaccacatgg
1621 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaac
1681 atgccaattt ccccaacaat atccagatag gggggttatt tccaaaccaa caatcacagg
1741 aacatgcggc ttttaggttt gctttgtcac aactcacgga gccccccaag ttgcttcccc
1801 agatcgatat tgtgaacatc agcgacagct ttgagatgac ttaccgtttc tgttcccagt
1861 tctccaaagg agtctatgcc atctttggat tttatgaacg aaggactgtc aacatgctga
1921 cctccttctg tggggccctc catgtgtgct tcattactcc aagttttcct gttgacacat
1981 ccaatcaatt tgtccttcag ctacgcccgg aactacagga agctctcatt agcattatcg
2041 accattacaa gtggcaaacc tttgtctaca tttatgatgc tgaccggggc ctgtcagtcc
2101 tgcagagagt cttggataca gccgcagaga agaactggca ggtaacggct gtcaacattc
2161 tgacaaccac cgaggaagga taccggatgc tctttcagga cctggagaag aaaaaggaga
2221 ggctggtggt ggttgactgt gaatcagaac gcctcaacgc catcctgggc cagatcgtga
2281 agctagaaaa gaatggcatc gggtaccact acatcctcgc caatctgggc ttcatggaca
2341 ttgacttaaa taagttcaag gagagcggag ccaatgtgac aggtttccag ctggtgaact
2401 acacagacac gatcccagcc agaatcatgc agcaatggag gacaagtgac tcccgagacc
2461 ataccagggt ggactggaag aggccaaagt acacttctgc tctcacctat gatggtgtca
2521 aggtgatggc tgaggccttc caaagcctgc ggaggcagag gattgacata tcccgccggg
2581 ggaatgctgg ggactgtctg gctaacccag ctgtgccctg gggtcaaggg atcgacatcc
2641 agagagccct gcagcaggtg cgcttcgaag gtttgacagg aaatgtgcag ttcaacgaga
2701 aagggcgccg gaccaattac accctccacg tgatcgaaat gaaacatgat ggaatccgaa
2761 agattggtta ctggaatgaa gacgataaat ttgtccccgc agccaccgac gctcaggctg
2821 gaggggacaa ctcaagcgtc cagaatagga cctacatcgt cactactatc ctcgaagatc
2881 cttacgtgat gcttaaaaag aatgccaacc agtttgaggg caatgaccgc tatgagggct
2941 actgtgtgga gctggctgca gagatcgcca agcacgtggg ctactcctac cgacttgaga
3001 ttgtcagcga cggcaaatat ggagcccggg atcccgacac aaaggcttgg aatggcatgg
3061 tgggagaact ggtctatgga agagcagacg tggctgtggc tcccttgacc ataaccttgg
3121 tccgggagga agtcatcgac ttctccaagc cattcatgag tttgggaatc tccattatga
3181 ttaagaagcc acagaagtcc aagccaggtg tcttctcctt tcttgaccct ttggcctatg
3241 agatctggat gtgtatagtg tttgcctaca ttggagtgag cgtcgtcctc ttcctggtca
3301 gccgtttcag cccctacgaa tggcacagcg aagagtttga agagggacga gaccagacaa
3361 ccagtgacca gtcaaatgag tttggcatat tcaacagcct gtggttctcc ctgggggcct
3421 tcatgcagca aggatgtgac atttccccca ggtccctgtc cggacgcatc gtcggcggcg
3481 tctggtggtt cttcactttg atcatcatct cctcgtacac agccaacctg gctgccttcc
3541 tgactgtgga gaggatggtg tctcccattg agagtgcaga ggacctggca aagcagacgg
3601 aaattgctta tgggacattg gaagcaggct ccactaagga gttcttcagg agatctaaaa
3661 tcgctgtgtt tgagaaaatg tggacataca tgaagtctgc agaaccatcc gtgtttgttc
3721 ggaccacaga ggaaggcatg atcagagtga gaaaatctaa aggcaaatac gcctacctcc
3781 tggagtccac catgaatgag tatatcgagc aacgaaagcc ctgtgacacc atgaaagtgg
3841 gaggtaactt ggattccaaa ggctatggca ttgcgacacc caaggggtcc gccctgagaa
3901 atccagtaaa cctggcagtg ttaaaactga acgagcaggg gcttttggac aaattgaaaa
3961 acaaatggtg gtacgacaag ggcgagtgcg gcagcggggg aggtgactcc aaggacaaga
4021 ccagcgcttt gagcctcagc aatgtggcag gcgtgttcta catcctgatt ggagggctgg
4081 gactggccat gctggttgcc ttaatcgagt tctgctacaa atcccgtagc gagtcgaagc
4141 ggatgaaggg tttctgtttg atcccacagc aatccatcaa tgaagccata cggacatcga
4201 ccctcccccg gaacagtggg gcaggagcca gcggaggagg cggcagtgga gagaatggcc
4261 gggtggtcag ccaggacttc cccaagtcca tgcaatccat tccctgcatg agtcacagtt
4321 cagggatgcc cttgggagcc acaggattgt aactctagag gatcccgggt ggcatccctg
4381 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct
4441 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat
4501 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg
4561 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc
4621 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc
4681 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt
4741 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt
4801 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt taaaataact
4861 ataccagcag gaggacgtcc agacacagca taggctacct ggccatgccc aaccggtggg
4921 acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc agtagatgcc
4981 tgttgaattg ggtacgcggc cagcttggct gtggaatgtg tgtcagttag ggtgtggaaa
5041 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
5101 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
5161 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
5221 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
5281 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
5341 ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
5401 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
5461 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgct
5521 gcaggtcgac tctagggccg cgtaccgagc tcgaattccc tatagtgagt cgtattaaat
5581 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
5641 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
5701 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
5761 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
5821 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
5881 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
5941 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
6001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
6061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
6121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
6181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
6241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
6301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
6361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
6421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
6481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
6541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
6601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
6661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
6721 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
6781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
6841 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
6901 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
6961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
7021 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
7081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
7141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
7201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
7261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
7321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
7381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
7441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
7501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
7561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
7621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
7681 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
7741 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
7801 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
7861 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
7921 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
7981 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
8041 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
8101 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
8161 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
8221 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
8281 gggatgtgct gcaaggcgat taagttgggt aacg
//
